The effect of media and plant growth regulators on embryo culture of Salvia leriifolia:

Salvia leriifolia (Lamiaceae) is an endemic endangered plant of Khorasan and Semnan province from Iran. Poor germination of seeds for this plant is a serious problem tomass production. The first step to improve this precious plant is to produce sterile plantlets in order to prepare explants of appropriate vigor. In this study, in vitro culture of Salvia leriifolia embryo was performed through a factorial experiment in the form of completely random design including culture medium in 3 types (MS.1/2MS,B5), BAP of 4 levels(0,1,2,3 mgl -1 ), NAA at 3 levels(0,0.5,1 mgl -1 ) and 3 replications. Statistical analysis was performed according to the JMP software. The results showed that, MS and 1/2MS appeared to be more efficient than B5 medium and significant differences were observed. Efficient concentrations of BAP and NAA were 1 mg/lit and had a significant effect in regeneration and growth of embryos. So that the entire seedling were obtained ten days after planting .Direct regeneration with minimal Somaclonal variation through embryo culture in MS and 1/2MS media supplemented with 1mg/lit BAP and NAA asthe bestoptionforfast accessto resistant seedling totheusabilitygenetic engineering and biotechnology and propagation of Salvia leriifolia is recommended.